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il 17rb constructs  (OriGene)


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    OriGene il 17rb constructs
    ORF8 binding enhances the heterodimerization of hIL-17RA/C. (A) Purified ORF8-Flag interacts with hIL-17RA and hIL-17RC. At 24 h posttransfection of the IL-17 receptor construct, 293T cells were subsequently incubated for 30 min with mock-treated or purified ORF8-Flag, followed by staining with anti-Flag antibody for flow cytometry. (B) Purified ORF8-Flag protein interacts with endogenous IL-17RA and IL-17RC in THP-1 cells. THP-1 cells were incubated with purified ORF8-Flag for 2 h. Cell lysates were immunoprecipitated with anti-Flag, followed by immunoblotting with the indicated antibodies. (C) Effects of IL-17RA/B/C deficiency on the ORF8-induced phosphorylation of p65 and IκBα. IL-17RA, <t>IL-17RB,</t> or IL-17RC knockout (KO) THP-1 cells were generated by the CRISPR-Cas9 method. IL-17RA KO, IL-17RB KO, IL-17RC KO, or control THP-1 cells were mock treated or treated with ORF8 for the indicated times, followed by immunoblotting with the indicated antibodies. (D) Effects of IL-17RA/B/C deficiency on ORF8-mediated cytokine gene expression. IL-17RA KO, IL-17RB KO, IL-17RC KO, or control THP-1 cells were mock treated or treated with ORF8 for 16 h, followed by qRT-PCR to measure the mRNA levels of indicated genes. (E) Proximity ligation assay (PLA) of IL-17RA/C heterodimerization in NIH 3T3 cells. At 24 h posttransfection with HA-hIL-17RA and Myc-hIL-17RC, NIH 3T3 cells were treated with hIL-17A or purified ORF8 for 30 min, followed by PLA. P values were calculated by two-way ANOVA with Dunnett’s posttest in panel D or one-way ANOVA with Tukey’s posttest in panel E. *, P < 0.05; **, P < 0.01; ***, P < 0.005; ****, P < 0.0001.
    Il 17rb Constructs, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 17rb constructs/product/OriGene
    Average 93 stars, based on 1 article reviews
    il 17rb constructs - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "Viral Mimicry of Interleukin-17A by SARS-CoV-2 ORF8"

    Article Title: Viral Mimicry of Interleukin-17A by SARS-CoV-2 ORF8

    Journal: mBio

    doi: 10.1128/mbio.00402-22

    ORF8 binding enhances the heterodimerization of hIL-17RA/C. (A) Purified ORF8-Flag interacts with hIL-17RA and hIL-17RC. At 24 h posttransfection of the IL-17 receptor construct, 293T cells were subsequently incubated for 30 min with mock-treated or purified ORF8-Flag, followed by staining with anti-Flag antibody for flow cytometry. (B) Purified ORF8-Flag protein interacts with endogenous IL-17RA and IL-17RC in THP-1 cells. THP-1 cells were incubated with purified ORF8-Flag for 2 h. Cell lysates were immunoprecipitated with anti-Flag, followed by immunoblotting with the indicated antibodies. (C) Effects of IL-17RA/B/C deficiency on the ORF8-induced phosphorylation of p65 and IκBα. IL-17RA, IL-17RB, or IL-17RC knockout (KO) THP-1 cells were generated by the CRISPR-Cas9 method. IL-17RA KO, IL-17RB KO, IL-17RC KO, or control THP-1 cells were mock treated or treated with ORF8 for the indicated times, followed by immunoblotting with the indicated antibodies. (D) Effects of IL-17RA/B/C deficiency on ORF8-mediated cytokine gene expression. IL-17RA KO, IL-17RB KO, IL-17RC KO, or control THP-1 cells were mock treated or treated with ORF8 for 16 h, followed by qRT-PCR to measure the mRNA levels of indicated genes. (E) Proximity ligation assay (PLA) of IL-17RA/C heterodimerization in NIH 3T3 cells. At 24 h posttransfection with HA-hIL-17RA and Myc-hIL-17RC, NIH 3T3 cells were treated with hIL-17A or purified ORF8 for 30 min, followed by PLA. P values were calculated by two-way ANOVA with Dunnett’s posttest in panel D or one-way ANOVA with Tukey’s posttest in panel E. *, P < 0.05; **, P < 0.01; ***, P < 0.005; ****, P < 0.0001.
    Figure Legend Snippet: ORF8 binding enhances the heterodimerization of hIL-17RA/C. (A) Purified ORF8-Flag interacts with hIL-17RA and hIL-17RC. At 24 h posttransfection of the IL-17 receptor construct, 293T cells were subsequently incubated for 30 min with mock-treated or purified ORF8-Flag, followed by staining with anti-Flag antibody for flow cytometry. (B) Purified ORF8-Flag protein interacts with endogenous IL-17RA and IL-17RC in THP-1 cells. THP-1 cells were incubated with purified ORF8-Flag for 2 h. Cell lysates were immunoprecipitated with anti-Flag, followed by immunoblotting with the indicated antibodies. (C) Effects of IL-17RA/B/C deficiency on the ORF8-induced phosphorylation of p65 and IκBα. IL-17RA, IL-17RB, or IL-17RC knockout (KO) THP-1 cells were generated by the CRISPR-Cas9 method. IL-17RA KO, IL-17RB KO, IL-17RC KO, or control THP-1 cells were mock treated or treated with ORF8 for the indicated times, followed by immunoblotting with the indicated antibodies. (D) Effects of IL-17RA/B/C deficiency on ORF8-mediated cytokine gene expression. IL-17RA KO, IL-17RB KO, IL-17RC KO, or control THP-1 cells were mock treated or treated with ORF8 for 16 h, followed by qRT-PCR to measure the mRNA levels of indicated genes. (E) Proximity ligation assay (PLA) of IL-17RA/C heterodimerization in NIH 3T3 cells. At 24 h posttransfection with HA-hIL-17RA and Myc-hIL-17RC, NIH 3T3 cells were treated with hIL-17A or purified ORF8 for 30 min, followed by PLA. P values were calculated by two-way ANOVA with Dunnett’s posttest in panel D or one-way ANOVA with Tukey’s posttest in panel E. *, P < 0.05; **, P < 0.01; ***, P < 0.005; ****, P < 0.0001.

    Techniques Used: Binding Assay, Purification, Construct, Incubation, Staining, Flow Cytometry, Immunoprecipitation, Western Blot, Knock-Out, Generated, CRISPR, Expressing, Quantitative RT-PCR, Proximity Ligation Assay



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    il 17rb constructs  (OriGene)
    93
    OriGene il 17rb constructs
    ORF8 binding enhances the heterodimerization of hIL-17RA/C. (A) Purified ORF8-Flag interacts with hIL-17RA and hIL-17RC. At 24 h posttransfection of the IL-17 receptor construct, 293T cells were subsequently incubated for 30 min with mock-treated or purified ORF8-Flag, followed by staining with anti-Flag antibody for flow cytometry. (B) Purified ORF8-Flag protein interacts with endogenous IL-17RA and IL-17RC in THP-1 cells. THP-1 cells were incubated with purified ORF8-Flag for 2 h. Cell lysates were immunoprecipitated with anti-Flag, followed by immunoblotting with the indicated antibodies. (C) Effects of IL-17RA/B/C deficiency on the ORF8-induced phosphorylation of p65 and IκBα. IL-17RA, <t>IL-17RB,</t> or IL-17RC knockout (KO) THP-1 cells were generated by the CRISPR-Cas9 method. IL-17RA KO, IL-17RB KO, IL-17RC KO, or control THP-1 cells were mock treated or treated with ORF8 for the indicated times, followed by immunoblotting with the indicated antibodies. (D) Effects of IL-17RA/B/C deficiency on ORF8-mediated cytokine gene expression. IL-17RA KO, IL-17RB KO, IL-17RC KO, or control THP-1 cells were mock treated or treated with ORF8 for 16 h, followed by qRT-PCR to measure the mRNA levels of indicated genes. (E) Proximity ligation assay (PLA) of IL-17RA/C heterodimerization in NIH 3T3 cells. At 24 h posttransfection with HA-hIL-17RA and Myc-hIL-17RC, NIH 3T3 cells were treated with hIL-17A or purified ORF8 for 30 min, followed by PLA. P values were calculated by two-way ANOVA with Dunnett’s posttest in panel D or one-way ANOVA with Tukey’s posttest in panel E. *, P < 0.05; **, P < 0.01; ***, P < 0.005; ****, P < 0.0001.
    Il 17rb Constructs, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 17rb constructs/product/OriGene
    Average 93 stars, based on 1 article reviews
    il 17rb constructs - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    ORF8 binding enhances the heterodimerization of hIL-17RA/C. (A) Purified ORF8-Flag interacts with hIL-17RA and hIL-17RC. At 24 h posttransfection of the IL-17 receptor construct, 293T cells were subsequently incubated for 30 min with mock-treated or purified ORF8-Flag, followed by staining with anti-Flag antibody for flow cytometry. (B) Purified ORF8-Flag protein interacts with endogenous IL-17RA and IL-17RC in THP-1 cells. THP-1 cells were incubated with purified ORF8-Flag for 2 h. Cell lysates were immunoprecipitated with anti-Flag, followed by immunoblotting with the indicated antibodies. (C) Effects of IL-17RA/B/C deficiency on the ORF8-induced phosphorylation of p65 and IκBα. IL-17RA, IL-17RB, or IL-17RC knockout (KO) THP-1 cells were generated by the CRISPR-Cas9 method. IL-17RA KO, IL-17RB KO, IL-17RC KO, or control THP-1 cells were mock treated or treated with ORF8 for the indicated times, followed by immunoblotting with the indicated antibodies. (D) Effects of IL-17RA/B/C deficiency on ORF8-mediated cytokine gene expression. IL-17RA KO, IL-17RB KO, IL-17RC KO, or control THP-1 cells were mock treated or treated with ORF8 for 16 h, followed by qRT-PCR to measure the mRNA levels of indicated genes. (E) Proximity ligation assay (PLA) of IL-17RA/C heterodimerization in NIH 3T3 cells. At 24 h posttransfection with HA-hIL-17RA and Myc-hIL-17RC, NIH 3T3 cells were treated with hIL-17A or purified ORF8 for 30 min, followed by PLA. P values were calculated by two-way ANOVA with Dunnett’s posttest in panel D or one-way ANOVA with Tukey’s posttest in panel E. *, P < 0.05; **, P < 0.01; ***, P < 0.005; ****, P < 0.0001.

    Journal: mBio

    Article Title: Viral Mimicry of Interleukin-17A by SARS-CoV-2 ORF8

    doi: 10.1128/mbio.00402-22

    Figure Lengend Snippet: ORF8 binding enhances the heterodimerization of hIL-17RA/C. (A) Purified ORF8-Flag interacts with hIL-17RA and hIL-17RC. At 24 h posttransfection of the IL-17 receptor construct, 293T cells were subsequently incubated for 30 min with mock-treated or purified ORF8-Flag, followed by staining with anti-Flag antibody for flow cytometry. (B) Purified ORF8-Flag protein interacts with endogenous IL-17RA and IL-17RC in THP-1 cells. THP-1 cells were incubated with purified ORF8-Flag for 2 h. Cell lysates were immunoprecipitated with anti-Flag, followed by immunoblotting with the indicated antibodies. (C) Effects of IL-17RA/B/C deficiency on the ORF8-induced phosphorylation of p65 and IκBα. IL-17RA, IL-17RB, or IL-17RC knockout (KO) THP-1 cells were generated by the CRISPR-Cas9 method. IL-17RA KO, IL-17RB KO, IL-17RC KO, or control THP-1 cells were mock treated or treated with ORF8 for the indicated times, followed by immunoblotting with the indicated antibodies. (D) Effects of IL-17RA/B/C deficiency on ORF8-mediated cytokine gene expression. IL-17RA KO, IL-17RB KO, IL-17RC KO, or control THP-1 cells were mock treated or treated with ORF8 for 16 h, followed by qRT-PCR to measure the mRNA levels of indicated genes. (E) Proximity ligation assay (PLA) of IL-17RA/C heterodimerization in NIH 3T3 cells. At 24 h posttransfection with HA-hIL-17RA and Myc-hIL-17RC, NIH 3T3 cells were treated with hIL-17A or purified ORF8 for 30 min, followed by PLA. P values were calculated by two-way ANOVA with Dunnett’s posttest in panel D or one-way ANOVA with Tukey’s posttest in panel E. *, P < 0.05; **, P < 0.01; ***, P < 0.005; ****, P < 0.0001.

    Article Snippet: The pCMV6-IL-17RA and IL-17RB constructs were purchased from Origene.

    Techniques: Binding Assay, Purification, Construct, Incubation, Staining, Flow Cytometry, Immunoprecipitation, Western Blot, Knock-Out, Generated, CRISPR, Expressing, Quantitative RT-PCR, Proximity Ligation Assay